Perfil (CV) del personal docente investigador

Fernández Silva, Patricio
Departamento: Departamento de Bioquímica y Biología Molecular y Celular
Área: Bioquímica y Biología Molecular
Centro: Facultad de Ciencias

Research Institute: INSTITUTO DE BIOCOMPUTACIÓN Y FÍSICA DE SISTEMAS COMPLEJOS (BIFI)
Grupo: E35_23R: Grupo de referencia biología estructural
Categoría profesional: Prof. Titular Univ.

Cargos
  • Miembro del Equipo de Dirección sin Cargo Académico del Departamento de Bioquímica y Biología Molecular y Celular
Correo electrónico: pfsilva@unizar.es
ORCID: 0000-0001-8971-7355

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Artículos

  • Moreno-Loshuertos, Raquel; Fernández-Silva, Patricio. Isolation of Mitochondria for Mitochondrial Supercomplex Analysis from Small Tissue and Cell Culture Samples. JOURNAL OF VISUALIZED EXPERIMENTS : JOVE. 2024. DOI: 10.3791/66771

  • Soler-Agesta, Ruth; Marco-Brualla, Joaquín; Fernández-Silva, Patricio; Mozas, Pilar; Anel, Alberto; Moreno Loshuertos, Raquel. Transmitochondrial Cybrid Generation Using Cancer Cell Lines. JOURNAL OF VISUALIZED EXPERIMENTS : JOVE. 2023. DOI: 10.3791/65186

  • Gu, Y.; Piñol, R.; Moreno-Loshuertos, R.; Brites, C. D. S.; Zeler, J.; Martínez, A.; Maurin-Pasturel, G.; Fernández-Silva, P.; Marco-Brualla, J.; Téllez, P.; Cases, R.; Navarro Belsué, R.; Bonvin, D.; Carlos, L. D.; Millán, Á. Local temperature increments and induced cell death in intracellular magnetic hyperthermia. ACS NANO. 2023. DOI: 10.1021/acsnano.3c00388

  • Moreno-Loshuertos, Raquel; Marco-Brualla, Joaquín; Meade, Patricia; Soler-Agesta, Ruth; Enriquez, José A.; Fernández-Silva, Patricio. How hot can mitochondria be? Incubation at temperatures above 43 °C induces the degradation of respiratory complexes and supercomplexes in intact cells and isolated mitochondria. MITOCHONDRION. 2023. DOI: 10.1016/j.mito.2023.02.002

  • Gu, Yuanyu; Piñol, Rafael; Moreno-Loshuertos, Raquel; Brites, Carlos D.S.; Zeler, Justyna; Martínez, Abelardo; Maurin-Pasturel, Guillaume; Fernández-Silva, Patricio; Marco-Brualla, Joaquín; Téllez, Pedro; Cases, Rafael; Navarro Belsué, Rafael; Bonvin, Debora; Carlos, Luís D.; Millán, Angel. Reply to the Comment on “Local Temperature Increments and Induced Cell Death in Intracellular Magnetic Hyperthermia”. ACS NANO. 2023. DOI: 10.1021/acsnano.3c06965

  • Moreno-Loshuertos, Raquel; Movilla, Nieves; Marco-Brualla, Joaquín; Soler-Agesta, Ruth; Ferreira, Patricia; Enríquez, José Antonio; Fernández-Silva, Patricio. A Mutation in Mouse MT-ATP6 Gene Induces Respiration Defects and Opposed Effects on the Cell Tumorigenic Phenotype. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023. DOI: 10.3390/ijms24021300

  • Silva-Pinheiro, P.; Pardo-Hernández, C.; Reyes, A.; Tilokani, L.; Mishra, A.; Cerutti, R.; Li, S.; Rozsivalova, D.-H.; Valenzuela, S.; Dogan, S.A.; Peter, B.; Fernández-Silva, P.; Trifunovic, A.; Prudent, J.; Minczuk, M.; Bindoff, L.; Macao, B.; Zeviani, M.; Falkenberg, M.; Viscomi, C. DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion. NUCLEIC ACIDS RESEARCH. 2021. DOI: 10.1093/nar/gkab282

  • Silva-Pinheiro, Pedro; Pardo-Hernández, Carlos; Reyes, Aurelio; Tilokani, Lisa; Mishra, Anup; Cerutti, Raffaele; Li, Shuaifeng; Rozsivalova, Dieu-Hien; Valenzuela, Sebastian; Dogan, Sukru A; Peter, Bradley; Fernández-Silva, Patricio; Trifunovic, Aleksandra; Prudent, Julien; Minczuk, Michal; Bindoff, Laurence; Macao, Bertil; Zeviani, Massimo; Falkenberg, Maria; Viscomi, Carlo. Correction to ‘DNA polymerase gamma mutations that impair holoenzyme stability cause catalytic subunit depletion’. NUCLEIC ACIDS RESEARCH. 2021. DOI: 10.1093/nar/gkab837

  • Piñol, R.; Zeler, J.; Brites, C.D.S.; Gu, Y.; Téllez, P.; Carneiro Neto, A.N.; Da Silva, T.E.; Moreno-Loshuertos, R.; Fernandez-Silva, P.; Gallego, A.I.; Martinez-Lostao, L.; Martínez, A.; Carlos, L.D.; Millán, A. Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles. NANO LETTERS. 2020. DOI: 10.1021/acs.nanolett.0c02163

  • Delavallée, L.; Mathiah, N.; Cabon, L.; Mazeraud, A.; Brunelle-Navas, M.N.; Lerner, L.K.; Tannoury, M.; Prola, A.; Moreno-Loshuertos, R.; Baritaud, M.; Vela, L.; Garbin, K.; Garnier, D.; Lemaire, C.; Langa-Vives, F.; Cohen-Salmon, M.; Fernández-Silva, P.; Chrétien, F.; Migeotte, I.; Susin, S.A. Mitochondrial AIF loss causes metabolic reprogramming, caspase-independent cell death blockade, embryonic lethality, and perinatal hydrocephalus. MOLECULAR METABOLISM. 2020. DOI: 10.1016/j.molmet.2020.101027

  • Gu, Y.; Yoshikiyo, M.; Namai, A.; Bonvin, D.; Martinez, A.; Piñol, R.; Téllez, P.; Silva, N.J.O.; Ahrentorp, F.; Johansson, C.; Marco-Brualla, J.; Moreno-Loshuertos, R.; Fernández-Silva, P.; Cui, Y.; Ohkoshi, S.I.; Millán, A. Magnetic hyperthermia with e-Fe2O3 nanoparticles. RSC ADVANCES. 2020. DOI: 10.1039/d0ra04361c

  • Marco-Brualla, Joaquín; Al-Wasaby, Sameer; Soler, Ruth; Romanos, Eduardo; Conde, Blanca; Justo-Méndez, Raquel; Enríquez, José A.; Fernández-Silva, Patricio; Martínez-Lostao, Luis; Villalba, Martín; Moreno-Loshuertos, Raquel; Anel, Alberto. Mutations in the ND2 subunit of mitochondrial complex I are sufficient to confer increased tumorigenic and metastatic potential to cancer cells. CANCERS. 2019. DOI: 10.3390/cancers11071027

  • Hurtado-Roca, Y.; Ledesma, M.; Gonzalez-Lazaro, M.; Moreno-Loshuertos, R.; Fernandez-Silva, P.; Enriquez, J.A.; Laclaustra, M. Adjusting MtDNA quantification in whole blood for peripheral blood platelet and leukocyte counts. PLOS ONE. 2016. DOI: 10.1371/journal.pone.0163770

  • Guarás, A.; Perales-Clemente, E.; Calvo, E.; Acín-Pérez, R.; Loureiro-Lopez, M.; Pujol, C.; Martínez-Carrascoso, I.; Nuñez, E.; García-Marqués, F.; Rodríguez-Hernández, M.A.; Cortés, A.; Diaz, F.; Pérez-Martos, A.; Moraes, C.T.; Fernández-Silva, P.; Trifunovic, A.; Navas, P.; Vazquez, J.; Enríquez, J.A. The CoQH2/CoQ Ratio Serves as a Sensor of Respiratory Chain Efficiency. CELL REPORTS. 2016. DOI: 10.1016/j.celrep.2016.03.009

  • Latorre-Pellicer, A.; Moreno-Loshuertos, R.; Lechuga-Vieco, A.; Sánchez-Cabo, F.; Torroja, C.; Acín-Pérez, R.; Calvo, E.; Aix, E.; González-Guerra, A.; Logan, A.; Bernad-Miana, M.; Romanos, E.; Cruz, R.; Cogliati, S.; Sobrino, B.; Carracedo, Á.; Pérez-Martos, A.; Fernández-Silva, P.; Ruíz-Cabello, J.; Murphy, M.P.; Flores, I.; Vázquez, J.; Enríquez, J.A. Mitochondrial and nuclear DNA matching shapes metabolism and healthy ageing. NATURE. 2016. DOI: 10.1038/nature18618

  • Mantilla, B.S.; Paes, L.S.; Pral, E.M.F.; Martil, D.E.; Thiemann, O.H.; Fernández-Silva, P.; Bastos, E.L.; Silber, A.M. Role of ¿1-pyrroline-5-carboxylate dehydrogenase supports mitochondrial metabolism and host-cell invasion of Trypanosoma cruzi. JOURNAL OF BIOLOGICAL CHEMISTRY. 2015. DOI: 10.1074/jbc.M114.574525

  • Acín-Pérez, Rebeca; Carrascoso, Isabel; Baixauli, Francesc; Roche-Molina, Marta; Latorre-Pellicer, Ana; Fernández-Silva, Patricio; Mittelbrunn, María; Sanchez-Madrid, Francisco; Pérez-Martos, Acisclo; Lowell, Clifford a.; Manfredi, Giovanni; Enríquez, José antonio. ROS-triggered phosphorylation of complex II by Fgr kinase regulates cellular adaptation to fuel use. CELL METABOLISM. 2014. DOI: 10.1016/j.cmet.2014.04.015

  • Lapuente-Brun, E.; Moreno-Loshuertos, R.; Acín-Pérez, R.; Latorre-Pellicer, A.; Colas, C.; Balsa, E.; Perales-Clemente, E.; Quirós, P.M.; Calvo, E.; Rodríguez-Hernández, M.A.; Navas, P.; Cruz, R.; Carracedo, Á.; López-Otín, C.; Pérez-Martos, A.; Fernández-Silva, P.; Fernández-Vizarra, E.; Enríquez, J.A. Supercomplex assembly determines electron flux in the mitochondrial electron transport chain. SCIENCE. 2013. DOI: 10.1126/science.1230381

  • Moreno-Loshuertos, R.; Pérez-Martos, A.; Fernández-Silva, P.; Enríquez, Ja. Length variation in the mouse mitochondrial tRNAArg DHU loop size promotes OXPHOS functional differences. FEBS JOURNAL. 2013. DOI: 10.1111/febs.12466

  • Cogliati, Sara; Frezza, Christian; Soriano, Maria eugenia; Varanita, Tatiana; Quintana-Cabrera, Ruben; Corrado, Mauro; Cipolat, Sara; Costa, Veronica; Casarin, Alberto; Gomes, Ligia c.; Perales-Clemente, Ester; Salviati, Leonardo; Fernandez-Silva, Patricio; Enriquez, Jose a.; Scorrano, Luca. Mitochondrial cristae shape determines respiratory chain supercomplexes assembly and respiratory efficiency. CELL. 2013. DOI: 10.1016/j.cell.2013.08.032

  • Bayona-Bafaluy, M.;Sánchez-Cabo, F.;Fernández-Silva, P.;Pérez-Martos, A. ;Enríquez, J. A. A genome-wide shRNA screen for new OxPhos related genes. MITOCHONDRION. 2011. DOI: 10.1016/j.mito.2011.01.007

  • Perales-Clemente, E.;Fernández-Silva, P.;Acín-Pérez, R.;Pérez-Martos, A. ;Enríquez, J. A. Allotopic expression of mitochondrial-encoded genes in mammals: Achieved goal, undemonstrated mechanism or impossible task?. NUCLEIC ACIDS RESEARCH. 2011. DOI: 10.1093/nar/gkq769

  • Moreno-Loshuertos, R.;Ferrín, G.;Acín-Pérez, R.;Gallardo, M. E.;Viscomi, C.;Pérez-Martos, A.;Zeviani, M.;Fernández-Silva, P. ;Enríquez, J. A. Evolution meets disease: Penetrance and functional epistasis of mitochondrial tRNA mutations. PLOS GENETICS. 2011. DOI: 10.1371/journal.pgen.1001379

  • Fernández-Vizarra, E.;Enríquez, J. A.;Pérez-Martos, A.;Montoya, J. ;Fernández-Silva, P. Tissue-specific differences in mitochondrial activity and biogenesis. MITOCHONDRION. 2011. DOI: 10.1016/j.mito.2010.09.011

  • Perales-Clemente, E.;Fernandez-Vizarra, E.;Acin-Perez, R.;Movilla, N.;Bayona-Bafaluy, M. P.;Moreno-Loshuertos, R.;Perez-Martos, A.;Fernandez-Silva, P. ;Enriquez, J. A. Five entry points of the mitochondrially encoded subunits in mammalian complex I assembly. MOLECULAR AND CELLULAR BIOLOGY. 2010

  • Fernandez-Vizarra, E.;Ferrin, G.;Perez-Martos, A.;Fernandez-Silva, P.;Zeviani, M. ;Enriquez, J. A. Isolation of mitochondria for biogenetical studies: An update. MITOCHONDRION. 2010

  • Enriquez, J. A.;Acin-Perez,R.;Fernandez-Silva,P.;Perez-Martos,A. Respiratory Active Mitochondrial Supercomplexes. FEBS JOURNAL. 2009

  • Garrido, N.;Perez-Martos, A.;Faro, M.;Lou-Bonafonte, J.;Fernandez-Silva, P.;Lopez-Perez, M.;Montoya, J.;Enriquez, J. A. Cisplatin-Mediated Impairment of Mitochondrial DNA Metabolism Inversely Correlates with Glutathione Levels. BIOCHEMICAL JOURNAL. 2008

  • Perales-Clemente, E;Pilar Bayona-Bafaluy, M;Perez-Martos, A;Barrientos, A;Fernandez-Silva, P;Antonio Enriquez, J. Restoration of Electron Transport without Proton Pumping in Mammalian Mitochondria. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008

  • Acin-Perez, R.;Fernandez-Silva,P.;Peleato,M. Luisa;Perez-Martos,A.;Enriquez,J. A. Respiratory Active Mitochondrial Supercomplexes. MOLECULAR CELL. 2008

  • Fernandez-Vizarra, E.;Enriquez,J. A.;Perez-Martos,A.;Montoya,J.;Fernandez-Silva,P. Mitochondrial Gene Expression is Regulated at Multiple Levels and Differentially in the Heart and Liver by Thyroid Hormones. CURRENT GENETICS. 2008

  • Fernandez-Silva, P.;Acin-Perez,R.;Fernandez-Vizarra,E.;Perez-Martos,A.;Enriquez,J. A. In Vivo and in Organello Analyses of Mitochondrial Translation. METHODS IN CELL BIOLOGY. 2007

  • Enriquez Ja; Moreno-Loshuertos R; Acin-Perez R; Movilla N; Gallardo Me; de Cordoba Sr; Perez-Martos A; Fernandez-Silva P. Reactive oxygen species and the segregation of mtDNA sequence variants. NATURE GENETICS. 2007

  • Moreno-Loshuertos, R;Acin-Perez, R;Fernandez-Silva, P;Movilla, N;Perez-Martos, A;de Cordoba, S.R;Gallardo, M.E;Enriquez, J.A. Differences in reactive oxygen species production explain the phenotypes associated with common mouse mitochondrial DNA variants. NATURE GENETICS. 2006

  • Roberti, M;Fernandez Silva, P;Polosa, P.L;Fernandez Vizarra, E;Bruni, F;Deceglie, S;Montoya, J;Gadaleta, M.N;Cantatore, P. In Vitro Transcription Termination Activity of the Drosophila Mitochondrial Dna-Binding Protein Dmttf. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2005

  • Acin Perez, R.;Bayona Bafaluy, M. P.;Fernandez Silva, P.;Moreno Loshuertos, R.;Perez Martos, A.;Bruno, C.;Moraes, C. T.;Enriquez, J. A. Respiratory Complex Iii Is Required to Maintain Complex I in Mammalian Mitochondria. MOLECULAR CELL. 2004

  • Acin Perez, R.;Bayona Bafaluy, M. P.;Bueno, M.;Machicado, C.;Fernandez Silva, P.;Perez Martos, A.;Montoya, J.;Lopez Perez, M. J.;Sancho, J.;Enriquez, J. A. An Intragenic Suppressor in the Cytochrome C Oxidase I Gene of Mouse Mitochondrial Dna. HUMAN MOLECULAR GENETICS. 2003

  • Fernandez Silva, P.;Enriquez, J. A.;Montoya, J. Replication and transcription of mammalian mitochondrial dna. EXPERIMENTAL PHYSIOLOGY. 2003

  • Bayona-Bafaluy, M. P.;Acin-Perez, R.;Mullikin, J. C.;Park, J. S.;Moreno-Loshuertos, R.;Hu, P. Q.;Perez-Martos, A.;Fernandez-Silva, P.;Bai, Y. D.;Enriquez, J. A. Revisiting the Mouse Mitochondrial Dna Sequence. NUCLEIC ACIDS RESEARCH. 2003. DOI: 10.1093/nar/gkg739

  • Bayona Bafaluy, M Pilar; Fernandez Silva, Patricio; Enriquez, Jose A. The thankless task of playing genetics with mammalian mitochondrial DNA: A 30-year review. MITOCHONDRION. 2002

  • Fernández-Silva, Patricio ; Loguercio-Polosa, Paola ; Roberti, Marina; Montoya, Julio; Cantatore, Palmiro Sea urchin mtDBP is a two-faced transcription termination factor with a biased polarity depending on the RNA polymerase. NUCLEIC ACIDS RESEARCH. 2001. DOI: 10.1093/nar/29.22.4736

  • Asin, J. ;Perez Martos, A. ;Fernandez Silva, P. ;Montoya, J. ;Andreu, A. L. Iron(II) induces changes in the conformation of mammalian mitochondrial DNA resulting in a reduction of its transcriptional rate. FEBS LETTERS. 2000

  • Chomyn,A.;Enriquez, Ja;Micol V.;Fernandez-Silva, P.;Attardi G. The Mitochondrial Myopathy, Encephalopathy, Lactic Acidosis, and Stroke-like Episode Syndrome-associated Human Mitochondrial tRNALeu(UUR) Mutation Causes Aminoacylation Deficiency and Concomitant Reduced Association of mRNA with Ribosomes. JOURNAL OF BIOLOGICAL CHEMISTRY. 2000

  • Enriquez, J.A.; Fernandez-Silva, P.; Montoya, J. Autonomous regulation in mammalian mitochondrial DNA transcription. BIOLOGICAL CHEMISTRY. 1999

  • Enriquez, J. A.;Fernandez-Silva, P.;Garrido-Perez, N.;Montoya, J. Direct regulation of mitochondrial RNA synthesis by thyroid hormone. MOLECULAR AND CELLULAR BIOLOGY. 1999

  • Petruzzella, V; Tiranti, V; Fernandez, P; Ianna, P; Carrozzo, R ; Zeviani, M Identification and characterization of human cDNAs specific to BCS1, PET112, SCO1, COX15, and COX11, five genes involved in the formation and function of the mitochondrial respiratory chain. GENOMICS. 1998. DOI: 10.1006/geno.1998.5580

  • Casari, G.; de Fusco, M.; Ciarmatori, S.; Zeviani, M.; Mora, M.; Fernandez, P.; de Michele, G.; Filla, A.; Cocozza, S.; Marconi, R.; Durr, A.; Fontaine, B.; Ballabio, A. Spastic paraplegia and OXPHOS impairment caused by mutations in paraplegin, a nuclear-encoded mitochondrial metalloprotease. CELL. 1998. DOI: 10.1016/S0092-8674(00)81203-9

  • Enriquez, J. A.;Fernandez Silva, P.;Perez Martos, A.;Lopez Perez, M. J.;Montoya, J. The synthesis of mRNA in isolated mitochondria can be maintained for several hours and is inhibited by high levels of ATP. EUROPEAN JOURNAL OF BIOCHEMISTRY. 1996

  • Enriquez, J. A.;Perez Martos, A.;Fernandez Silva, P.;Lopez Perez, M. J.;Montoya, J. Rna-Synthesis in Isolated-Mitochondria from Brain Cortex, Cerebellum and Stem - Evidence of Different Transcriptional Rates. INTERNATIONAL JOURNAL OF BIOCHEMISTRY. 1993

  • Enriquez, J. A.;Perez Martos, A.;Fernandez Silva, P.;Lopez Perez, M. J.;Montoya, J. Specific Increase of a Mitochondrial Rna Transcript in Chronic Ethanol-Fed Rats. FEBS LETTERS. 1992

  • Villalta, M.;Fernandez Silva, P.;Beltran, B.;Enguita, L.;Lopez Perez, M. J.;Montoya, J. Molecular Characterization and Cloning of Sheep Mitochondrial-Dna. CURRENT GENETICS. 1992

  • Corbaton, V.;Muiño, M. T.;Fernandez Silva, P.;Lopez Perez, M. J.;Montoya, J. Estimation of the Chloramphenicol and Cycloheximide Inhibition of Protein-Synthesis in Brain Cholinergic Synaptosomes. BRAIN RESEARCH. 1991

  • Corbaton, V.;Fernandez Silva, P.;Lopez Perez, M. J.;Montoya, J. Analysis of Polyadenylated Rna from Brain Synaptosomes and Mitochondria. NEUROCHEMICAL RESEARCH. 1990

Comunicaciones

  • Montoya, J.; Playan, A.; Alcaine, M.J.; Solano, A.; Fernandez Silva, P.; Enriquez, J.A. Avances recientes en genetica de las epilepsias. Genetica de las epilepsias mitocondriales. REVISTA DE NEUROLOGIA. 2000

Capítulos

  • Tissue specificity of energy metabolism in mitochondria. Moreno Loshuertos, Raquel; Fernández Silva, Patricio. CLINICAL BIOENERGETICS. 2020

  • Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides: a mutagenesis approach. Bayona-Bafaluy, María Pilar; Movilla, Nieves ; Pérez-Martos, Acisclo ; Fernández Silva, Patricio; Enriquez, José Antonio. MEMBRANE TRAFFICKING. METHODS IN MOLECULAR BIOLOGY. 2008

  • Isolation of mitochondria from mammalian tissues and cultured cells. Fernández-Vizarra, Erika; Fernández-Silva, Patricio; Enríquez, José Antonio. CELL BIOLOGY: A LABORATORY HANDBOOK. 3RD EDITION. 2006

  • Advances in mammalian mitochondria DNA transcription. Fernández-Vizarra, Erika; Fernández Silva, Patricio; López-Pérez, Manuel José; Montoya, Julio. NEW FRONTIERS IN MITOCHONDRIAL BIOGENESIS AND DISEASE. 2005



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